Prkcg ELISA Kit for Mouse(Mus musculus) | EIAab.comPrkcg ELISA Kit for Mouse(Mus musculus) | EIAab.com

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Prkcg (Gene name),

Protein kinase C gamma type (Protein name ), KPCG_MOUSE from NCBI database.

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General Annotation

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Antigen Annotation

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3D

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Predicted Eptitope

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Vaild Sequence

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Related Databases

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Gene name:

Prkcg(Pkcc;Pkcg;Prkcc);

Protein name:

Protein kinase C gamma type(PKC-gamma);

Alternative:

Organism:

Mouse (Mus musculus).

General Annotation

Sub Unit:

Interacts with CDCP1.

Function:

Calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. Serves as the receptor for phorbol esters, a class of tumor promoters. Phosphorylates a number of proteins involved in neuronal development and is required for stress responses in neurological tissue. Modulates cerebellar neural transmission. Required for the elimination of multiple climbing fibers during innervation of Purkinje cells in developing cerebellum.

Subcellular Location:

Cytoplasm Cell membrane Peripheral membrane protein Cell junction synapse synaptosome Translocates to synaptic membranes on stimulation.

Protein Attributes:

Sequence length:

697

Sequence:

50:

MAGLGPGGGD | SEGGPRPLFC | RKGALRQKVV | HEVKSHKFTA | RFFKQPTFCS |

100:

HCTDFIWGIG | KQGLQCQVCS | FVVHRRCHEF | VTFECPGAGK | GPQTDDPRNK |

150:

HKFRLHSYSS | PTFCDHCGSL | LYGLVHQGMK | CSCCEMNVHR | RCVRSVPSLC |

200:

GVDHTERRGR | LQLEIRAPTS | DEIHITVGEA | RNLIPMDPNG | LSDPYVKLKL |

250:

IPDPRNLTKQ | KTKTVKATLN | PVWNETFVFN | LKPGDVERRL | SVEVWDWDRT |

300:

SRNDFMGAMS | FGVSELLKAP | VDGWYKLLNQ | EEGEYYNVPV | ADADNCSLLQ |

350:

KFEACNYPLE | LYERVRMGPS | SSPIPSPSPS | PTDSKRCFFG | ASPGRLHISD |

400:

FSFLMVLGKG | SFGKVMLAER | RGSDELYAIK | ILKKDVIVQD | DDVDCTLVEK |

450:

RVLALGGRGP | GGRPHFLTQL | HSTFQTPDRL | YFVMEYVTGG | DLMYHIQQLG |

500:

KFKEPHAAFY | AAEIAIGLFF | LHNQGIIYRD | LKLDNVMLDA | EGHIKITDFG |

550:

MCKENVFPGS | TTRTFCGTPD | YIAPEIIAYQ | PYGKSVDWWS | FGVLLYEMLA |

600:

GQPPFDGEDE | EELFQAIMEQ | TVTYPKSLSR | EAVAICKGFL | TKHPGKRLGS |

650:

GPDGEPTIRA | HGFFRWIDWE | RLERLEIAPP | FRPRPCGRSG | ENFDKFFTRA |

697:

APALTPPDRL | VLASIDQADF | QGFTYVNPDF | VHPDARSPTS | PVPVPVM

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

UniGene:

String:

KEGG:

SMR:

Pfam:

Uniprot:

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ELISA Kit for Mouse PKC-gamma

Cat.:

E8823h

Price:

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MSDS:

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96T

Range:

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ELISA Kit for Mouse PKC-gamma

MSDS:

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ELISA Kit for Mouse PKC-gamma

Cat.:

E8823m

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MSDS:

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Packing:

96T

ELISA Kit for Mouse PKC-gamma

Cat.:

E8823r

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MSDS:

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Packing:

96T

ELISA Kit for Mouse PKC-gamma

MSDS:

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CLIA Kit for Mouse PKC-gamma

Cat.:

U8823h

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Looking for manual.

MSDS:

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96T

Range:

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CLIA Kit for Mouse PKC-gamma

MSDS:

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CLIA Kit for Mouse PKC-gamma

Cat.:

U8823m

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MSDS:

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Packing:

96T

CLIA Kit for Mouse PKC-gamma

Cat.:

U8823b

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MSDS:

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Packing:

96T

CLIA Kit for Mouse PKC-gamma

Cat.:

U8823r

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Packing:

96T

Polyclonal Antibody for Mouse PKC-gamma

Polyclonal Antibody for Mouse PKC-gamma

Cat.:

P8823Rb-m

Price:

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Packing:

40ug/0.2ml

Polyclonal Antibody for Mouse PKC-gamma

Polyclonal Antibody for Mouse PKC-gamma

Polyclonal Antibody for Mouse PKC-gamma

Cat.:

P8823Rb-h

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Packing:

40ug/0.2ml

Monoclonal Antibody for Mouse PKC-gamma

Monoclonal Antibody for Mouse PKC-gamma

Monoclonal Antibody for Mouse PKC-gamma

Monoclonal Antibody for Mouse PKC-gamma

Monoclonal Antibody for Mouse PKC-gamma

Protein for Mouse PKC-gamma

Protein for Mouse PKC-gamma

Protein for Mouse PKC-gamma

Protein for Mouse PKC-gamma

Protein for Mouse PKC-gamma

R&D Technical Data

s

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s

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Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.7%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.8%

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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

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Recovery

Recovery was determined by spiking various levels of PKCG into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	93-105
Plasma	98	94-106

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Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

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Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PKCG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	94-103%	93-106%	96-102%	91-107%
EDTA plasma(n=5)	92-104%	90-108%	92-104%	94-104%
heparin plasma(n=5)	95-103%	91-106%	93-102%	96-100%

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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

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References

1.

"Isolation and sequence of a mouse brain cDNA coding for protein kinase C-gamma isozyme."
Bowers B.J. , Parham C.L. , Sikela J.M. , Wehner J.M.
Gene123:263-265(1993) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
tissue: Brain.
2.

Tseng C.P. , Verma A.
Submitted (1994-01) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
strain: BALB/c.
tissue: Brain.
3.

"Modified hippocampal long-term potentiation in PKC gamma-mutant mice."
Abeliovich A. , Chen C. , Goda Y. , Silva A.J. , Stevens C.F. , Tonegawa S.
Cell75:1253-1262(1993) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN LONG-TERM POTENTIATION
4.

"Impaired motor coordination correlates with persistent multiple climbing fiber innervation in PKC gamma mutant mice."
Chen C. , Kano M. , Abeliovich A. , Chen L. , Bao S. , Kim J.J. , Hashimoto K. , Thompson R.F. , Tonegawa S.
Cell83:1233-1242(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: DISRUPTION PHENOTYPE
5.

"Preserved acute pain and reduced neuropathic pain in mice lacking PKCgamma."
Malmberg A.B. , Chen C. , Tonegawa S. , Basbaum A.I.
Science278:279-283(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN NEUROPATHIC PAIN
6.

"Enhanced mu-opioid responses in the spinal cord of mice lacking protein kinase Cgamma isoform."
Narita M. , Mizoguchi H. , Suzuki T. , Narita M. , Dun N.J. , Imai S. , Yajima Y. , Nagase H. , Suzuki T. , Tseng L.F.
J. Biol. Chem.276:15409-15414(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN OPIOID SIGNALING
7.

"Involvement of spinal protein kinase Cgamma in the attenuation of opioid mu-receptor-mediated G-protein activation after chronic intrathecal administration of [D-Ala2,N-MePhe4,Gly-Ol(5)]enkephalin."
Narita M. , Mizoguchi H. , Narita M. , Nagase H. , Suzuki T. , Tseng L.F.
J. Neurosci.21:3715-3720(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN OPIOID SIGNALING
8.

"Involvement of protein kinase Cgamma isoform in morphine-induced reinforcing effects."
Narita M. , Aoki T. , Ozaki S. , Yajima Y. , Suzuki T.
Neuroscience103:309-314(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN OPIOID SIGNALING
9.

"Reduced development of tolerance to the analgesic effects of morphine and clonidine in PKC gamma mutant mice."
Zeitz K.P. , Malmberg A.B. , Gilbert H. , Basbaum A.I.
Pain94:245-253(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION IN OPIOID SIGNALING
10.

"Abnormal features in mutant cerebellar Purkinje cells lacking junctophilins."
Ikeda A. , Miyazaki T. , Kakizawa S. , Okuno Y. , Tsuchiya S. , Myomoto A. , Saito S.Y. , Yamamoto T. , Yamazaki T. , Iino M. , Tsujimoto G. , Watanabe M. , Takeshima H.
Biochem. Biophys. Res. Commun.363:835-839(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT THR-514; THR-655 AND THR-674;SUBCELLULAR LOCATION;FUNCTION;TISSUE SPECIFICITY
11.

"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations."
Munton R.P. , Tweedie-Cullen R. , Livingstone-Zatchej M. , Weinandy F. , Waidelich M. , Longo D. , Gehrig P. , Potthast F. , Rutishauser D. , Gerrits B. , Panse C. , Schlapbach R. , Mansuy I.M.
Mol. Cell. Proteomics6:283-293(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Brain cortex.
12.

"Isoflurane inhibits protein kinase Cgamma and calcium/calmodulin dependent protein kinase ii-alpha translocation to synaptic membranes in ischemic mice brains."
Matsumoto S. , Murozono M. , Nagaoka D. , Matsuoka S. , Takeda A. , Narita H. , Watanabe S. , Isshiki A. , Watanabe Y.
Neurochem. Res.33:2302-2309(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION
13.

"PKCgamma participates in food entrainment by regulating BMAL1."
Zhang L. , Abraham D. , Lin S.T. , Oster H. , Eichele G. , Fu Y.H. , Ptacek L.J.
Proc. Natl. Acad. Sci. U.S.A.109:20679-20684(2012) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INTERACTION WITH ARNTL;DISRUPTION PHENOTYPE;TISSUE SPECIFICITY;PHOSPHORYLATION AT THR-655

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